Figure 3From: Time-dependent changes in the microenvironment of injured spinal cord affects the therapeutic potential of neural stem cell transplantation for spinal cord injurySurvival rate and differentiation phenotype of grafted cells in the sub-acute and chronic TP groups. A, Representative in vivo BLI of naïve mice transplanted with various numbers of NS/PCs. B, The number of NS/PCs and the measured photon count were significantly correlated in vivo. Values are means ± SEM (n = 3). C, Representative in vivo BLI of the sub-acute and chronic TP groups immediately and 42 days after transplantation. D, Quantitative analysis using BLI revealed no significant difference in the survival rate of grafted cells between the sub-acute and chronic TP groups up to 42 days after transplantation. Values are means ± SEM (n = 10). E, GFP+ grafted cells differentiated into Hu+ neurons, GFAP+ astrocytes, and APC+ oligodendrocytes in the sub-acute and chronic TP groups. F, The differentiation rate of grafted cells into the three neural cell lineages did not significantly differ between the sub-acute and chronic TP groups 42 days after transplantation. Only a small number of nestin+ neural progenitor cells was detected in the sub-acute and chronic TP groups. Values are means ± SEM (n = 3). Scale bar: 10 μm in (C).Back to article page