Figure 5

Histological and immunohistochemical analyses of brain tissues. (A) Histological observations in the hippocampus (CA1 region) and visual cortex. Paraffin sections of brain tissues were stained with Kluver-Barrera (KB) stain. (B) Immunohistochemical observations in visual cortex and DG. Frozen sections of brain tissues were stained with anti-α-CaMKII (green) or anti-p-α-CaMKII (red) antibody, and with DAPI (blue). a-CaMKII and DAPI signals, or p-α-CaMKII and DAPI signals, were respectively merged. (C) Amplified images and quantification of α-CaMKII and p-α-CaMKII in visual cortex and DG areas. A part of each image in Figure 5B was representation as amplified images. In the graphic representation, quantification of α-CaMKII and p-α-CaMKII positive signals in visual cortex and DG of B6 (white bar), low group (black bar), and high group (gray bar) were shown (a-CaMKII in visual cortex: P = 0.5333 in B6 vs low group, P < 0.05 in B6 vs high group, and P < 0.05 in low vs high groups; p-a-CaMKII in visual cortex: P = 0.2884 in B6 vs low group, P < 0.02 in B6 vs high group, and P < 0.0001 in low vs high groups; a-CaMKII of DG: P = 0.9775 in B6 vs low group, P < 0.02 in B6 vs high group, and P < 0.02 in low vs high groups; p-a-CaMKII of DG: P = 0.9263 in B6 vs low group, P < 0.05 in B6 vs high group, and P < 0.02 in low vs high groups). Values are the means ± SE. Asterisks indicate significant differences. (D) Immunohistochemical observations in the hippocampus (CA1 region). Paraffin sections of brain tissues from B6, low group, and high group mice were stained with anti-NMDA receptor 1 antibody. All scale bars, 50 µm.