Figure 6

Quantification of α-CaMKII protein and mRNA in visual cortex. (A) Quantification of α-CaMKII protein in the visual cortex by Western blotting analysis. Extracted protein of the visual cortex from B6, low group, and high group were blotted with an anti-α-CaMKII antibody. Bars indicate values of α-CaMKII protein expression in the visual cortex from the B6 (white bar), low group (black bar), and high group (gray bar). In the high group, remarkable downregulation of α-CaMKII proteins (P = 0.5148 B6 vs low group, P < 0.005 B6 vs high group, P < 0.005 low group vs high group) was observed. The β-actin was used as a loading control in the Western blotting. Values are the means ± SE. Asterisks indicate significant differences. (B) Quantification of α-CaMKII mRNA in the visual cortex. Total mRNAs were extracted from frozen samples of the visual cortex from B6, low group and high group, and cDNAs were obtained by a reverse transcription method. Relative expression levels of α-CaMKII mRNA were determined by a real-time monitoring PCR technique. Bars indicate values of relative expression levels of α-CaMKII mRNA in the B6 (white bar), low group (black bar), and high group (gray bar). The β-actin was used as a loading control in the real-time monitoring PCR technique. There was no significant difference among the three groups (P = 0.9972 B6 vs low group, P = 0.6214 B6 vs high group, P = 0.6238 low group vs high group). Values are means ± SE.