Tryptophan 2,3-dioxygenase is a key modulator of physiological neurogenesis and anxiety-related behavior in mice
© Kanai et al; licensee BioMed Central Ltd. 2009
Received: 20 January 2009
Accepted: 27 March 2009
Published: 27 March 2009
Although nutrients, including amino acids and their metabolites such as serotonin (5-HT), are strong modulators of anxiety-related behavior, the metabolic pathway(s) responsible for this physiological modulation is not fully understood. Regarding tryptophan (Trp), the initial rate-limiting enzymes for the kynurenine pathway of tryptophan metabolism are tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO). Here, we generated mice deficient for tdo (Tdo-/-). Compared with wild-type littermates, Tdo-/- mice showed increased plasma levels of Trp and its metabolites 5-hydroxyindoleacetic acid (5-HIAA) and kynurenine, as well as increased levels of Trp, 5-HT and 5-HIAA in the hippocampus and midbrain. These mice also showed anxiolytic modulation in the elevated plus maze and open field tests, and increased adult neurogenesis, as evidenced by double staining of BrdU and neural progenitor/neuronal markers. These findings demonstrate a direct molecular link between Trp metabolism and neurogenesis and anxiety-related behavior under physiological conditions.
Here, to better understand the metabolic pathways and enzymes responsible for anxiety-related behavior, we generated Tdo knock-out (Tdo-/-) mice and assessed the role of TDO in anxiety-related behavior and neurogenesis, and in systemic and brain Trp metabolism.
Generation of mice with targeted disruption of the tdo gene locus
We first disrupted the tdo gene in mice by homologous recombination. The targeting vector was constructed by replacing genomic tdo exons 1 and 2 (containing the translational initiation site) with the PGK-neomycin (Neo) cassette (Figure 1B). Heterozygous mice were crossed with C57BL/6 mice for five generations. Interbreeding of the resultant heterozygotes produced wild-type (Tdo+/+), heterozygote (Tdo+/-), and homozygote (Tdo-/-) mice, as identified by Southern blot analysis of Pvu II-digested genomic tail DNA (Figure 1C). The disruption of tdo was verified by the absence of tdo mRNA transcripts and TDO protein in the liver, as assessed by quantitative real-time PCR and Western blot analyses, respectively (Figure 1D and 1E). The null mutation of tdo was also verified by enzyme activity assays of liver extracts (Figure 1F). These mutant mice were born at ratios that followed Mendelian inheritance and matured for at least one year without apparent gross abnormalities. As TDO is predominantly expressed in the liver, we briefly looked at gross liver morphology: although little difference between 13-week-old Tdo-/- and Tdo+/+ mice was seen, confirmation of a lack of effect on the histological status of the liver will require further detailed examination.
Marked increase in plasma Trp and altered plasma Trp metabolite levels in Tdo-/- adult mice
We then examined whether levels of the major Trp metabolites kynurenine (Kyn) and kynurenic acid (KYNA) were reduced by tdo deletion, on the basis that the marked elevation of Trp in Tdo-/- mice suggested the insufficient compensatory conversion of Trp to formylkynurenine by IDO. Kyn levels were 2-fold higher in Tdo-/- than Tdo+/+ mice (Figure 2F), whereas KYNA levels showed no significant difference (Figure 2G).
Enhanced brain 5-HT synthesis in adult Tdo-/- mice
Modulation of anxiety-related behavior in 13- to 15-week-old Tdo-/- mice
We next assessed whether TDO deletion modulated anxiety-related behavior. There were a number of rationales for this: changes in levels of Trp and its metabolite 5-HT modulate either or both anxiety- and depression-related behavior [20, 21]; the important contribution proposed for Trp and 5-HT dysregulation in many psychiatric disorders, including anxiety, depression, schizophrenia, aggression, autism and alcoholism ; the possibility that TDO is directly involved in several psychiatric conditions, as evidenced by the potential association of human TDO2 gene polymorphisms with psychiatric diseases, such as Tourette syndrome, depression, and autism [13, 14]; and the possibility that familial hypertryptophanemia, with its associated mood abnormalities, is due to an inborn error in the normal conversion of Trp to Kyn, albeit that the causal gene for this disease has not yet been identified . Results showed that brain levels of Trp and 5-HT  were markedly elevated in Tdo-/- mice (Figure 3).
Increased proliferation of neural progenitors in the dentate gyrus of the hippocampus in 13-week-old Tdo-/- mice
Quantitative analysis of proliferating neural progenitors in SGZ 24 h after BrdU injection.
2.78 ± 0.23
5.31 ± 0.49
4.20 ± 0.45
0.80 ± 0.08
2.38 ± 0.13
6.90 ± 0.26*
6.07 ± 0.48*
1.49 ± 0.13*
0.58 ± 0.12
0.34 ± 0.06
0.42 ± 0.08
0.77 ± 0.12
1.15 ± 0.13*
0.47 ± 0.05*
0.93 ± 0.09*
1.27 ± 0.11*
Quantitative analysis of adult neurogenesis in SGZ 28 days after BrdU injection.
91.28 ± 1.30
2.75 ± 0.33
0.47 ± 0.12
0.64 ± 0.12
89.62 ± 0.87
5.16 ± 0.47*
1.14 ± 0.12*
1.00 ± 0.13*
Increased proliferation of neural progenitors in the subventricular zone and marked reduction in size of the lateral ventricles in 13-week-old Tdo-/- mice
To explore this possibility, BrdU (4 × 75 mg/kg) was injected into 13-week-old mice, which were then examined 24 h later. On TO-PRO-3 iodide (nuclear) staining, markedly more cells were seen in the SVZ of Tdo-/- than Tdo+/+ mice, particularly in the region surrounded by corpus callous (CC), striatum (Str) and LV, (Figure 6Ca and 6Cb). Further, Tdo-/- mice also showed more BrdU-positive cells in this region (Figure 6Cc–f) than wild-type mice. Immunostaining for nestin (red) or nestin plus BrdU (green, overlay view) in Tdo-/- mice revealed noticeably more nestin-positive neural stem cells in the SVZ, not only on the Str but also on the CC side (Figure 6D), suggesting marked neural stem cell proliferation in the SVZ. With regard to neural progenitor (migrating neuroblast) cells, immunostaining for DCX (red) and BrdU (green, overlay view) revealed a perceptible increase in the number of proliferating DCX-positive neural progenitor cells in the SVZ of Tdo-/- mice (Figure 6E). Similar results were obtained using PSA-NCAM as an additional marker for neural progenitor cells (Figure 6F). Taken together, these findings demonstrate that the loss of TDO induces the proliferation of both neural progenitors and neural stem cells in the SVZ, and hence might contribute, either fully or partly, to a decrease in the size of the LV.
Accelerated adult neurogenesis in the GCL of the olfactory bulb in 13-week-old Tdo-/- mice
Adult-born cells in the SVZ migrate along the rostral migratory stream to the olfactory bulb (OB) where they differentiate into interneurons. To determine if the number of newly generated neurons migrating to their final destination in the OB was altered in Tdo-/- mice, BrdU was administered to 9-week-old mice, which were sacrificed 4 weeks later. Although Cresyl violet-stained brain sections showed little difference between genotypes in the appearance of the OB (see additional file 1, Figure S2A), the estimated number of BrdU-positive cells in the GCL of the OB was 1.6-fold higher in Tdo-/- than Tdo+/+ mice (see additional file 1, Figure S2C and S2E, middle panels). These mice also showed an increase in the number of BrdU/PSA-NCAM double-positive proliferating neuroblasts, presumably derived from the SVZ (see additional file 1, Figure S2B and S2C, right panels); and a notable increase in the number of BrdU-positive cells co-labeled with NeuN in the GCL (see additional file 1, Figure S2D and S2E, right panel). These findings indicate that the enhanced proliferation of neural progenitors in the SVZ increased the number of migrating neuroblasts and enhanced adult neurogenesis in the GCL in the OB of 13-week-old Tdo-/- mice. Total PSA-NCAM- and NeuN-positive cell numbers of olfactory bulb were not altered in Tdo-/- mice in a similar manner with dentate gyrus, raising the concern that there is different survival rate of new born neural progenitors and neurons between wild type and Tdo-/- mice.
Using mice deficient for tdo, we provide the first evidence that TDO, one of two initial and rate-limiting enzymes for the kynurenine pathway of Trp metabolism, is directly linked to systemic Trp metabolism, neurogenesis and anxiety-related behavior in vivo. These mice had markedly increased plasma levels of Trp, 5-HIAA, ILA and IAA in the presence of IDO, demonstrating that the accumulation of Trp and acceleration of serotonergic and transamination pathways are largely dependent on TDO (Figure 1 and 2). Although other tryptophan metabolic enzymes, such as kynurenine formamidase, may also play a role in the regulation of systemic Trp levels and several important neural functions [16, 25–27], we propose that the greater severity of biochemical changes in our Tdo-/- mice, particularly in systemic Trp levels, than in other animal models indicates that TDO is the key regulatory enzyme in the modulation of systemic Trp levels. In addition to Trp, 5-HT and 5-HIAA levels were also elevated in the hippocampus and midbrain of these mice. Taken together, these findings demonstrate that TDO, which is expressed predominantly in the liver, plays an essential and dominant role in the in vivo regulation of brain levels of 5-HT, even in the presence of tryptophan hydroxylase-1 (in the periphery) and tryptophan hydroxylase-2 (in the brain). This finding contrasts with those of previous studies, which have indicated the latter two enzymes as rate-limiting for 5-HT synthesis [6, 28].
In contrast to our findings for Trp, plasma levels of Kyn and KYNA, which are downstream Trp metabolites generated by TDO (Figure 1), were sustained at physiological levels despite the absence of TDO. This finding suggests the presence of compensatory mechanisms to maintain Kyn and KYNA levels in Tdo-/- mice; and given that IDO mediates the same metabolic processes as TDO in various tissues, it is the most likely candidate. On this basis, IDO would be expected to decrease plasma and brain Trp levels but increase its downstream metabolites Kyn and KYNA . Our assessment of both TDO and IDO enzyme activities from liver lysates, however, showed a loss in the conversion of Trp to Kyn in Tdo-/- compared with Tdo+/+ mice (Figure 1F), suggesting that the compensatory mechanism(s) may function in extra-hepatic tissues and, in part, play a role in decreasing Trp level and increasing Kyn level. In addition, a modulatory mechanism(s) in Kyn pathway may also play a role. Increasing Trp concentrations in food decrease the enzymatic activity of quinolinate phosphoribosyltransferase (QPRT), a downstream metabolic enzyme of TDO . Although the mechanism remains unclear, our findings thus raise the possibility that Trp metabolism downstream of Kyn and KYNA plays a role in maintaining plasma Kyn and KYNA levels in Tdo-/- mice.
We also used these Tdo-/- mice to evaluate the role of TDO in anxiety-related behavior. Although the mechanism remains to be elucidated, TDO deletion had clear anxiolytic effects, as revealed by two classical behavioral tests. In agreement with our data, Yamasaki et al. reported marked reduction of the level of tdo mRNA in the hippocampus of alpha-CaMKII deficient mice (alpha-CaMKII+/-) that show anxiolytic phenotype . Trp and its catabolite 5-HT are thought to modulate mood control . Given that the roles of 5-HT1A receptor and 5-HT transporter during development in anxiety-related behavior have been reported, respectively [32, 33], it is postulated that Tdo-/- mice show anxiolytic change due to 5-HT-upregulation in the brain and subsequent modulation of neural development. In addition, in the adult, given postulation that 5-HT/5-HT1A receptor-mediated neurogenesis is critically involved in the anxiolytic effects of anti-depressant fluoxetine , one likely mechanism of this is that the deletion of hepatic TDO modulates plasma Trp and subsequently increases brain Trp and 5-HT, which in turn accelerates neurogenesis in the hippocampus. It should be noted that tdo and its variants mRNAs are expressed in various regions of developing and adult brain , suggesting a possible role of locally expressed TDOs in the brain for the specific regional modulation of the brain and subsequent behavioral modulation. Moreover, altered immunoreactivity against TDO has been reported in patients with schizophrenia and depression . In addition, given findings of a correlation between Kyn levels and the regulation of behavior in insects and of an increase in plasma Kyn concentration in endogenous anxiety in humans, we cannot exclude the possibility that anxiety-related behavior is also modulated by TDO-induced changes in Kyn, and possibly in other kynurenines as well [36, 37]. If the contribution of alterations in Kyn to anxiety-related behavior is indeed important, then TDO would appear to be a key modulator of this behavior under physiological conditions via the control of both 5-HT and Kyn. This possible role of TDO stands in contrast to that of TPH, which has been considered a rate-limiting enzyme in the synthesis of 5-HT but not of Kyn.
The role of stress and stress-induced glucocorticoids in affecting mood and anxiety is well known. Administration of glucocorticoids to rats results in elevations of the tryptophan-metaboliting enzymes and TPH in vivo and that administration of dexamethasone phosphate regulates TDO activity in cells from control and adrenarectomized mice, respectively [38, 39]. In addition, glucocorticoids regulate either or both the activity and mRNA levels of TDO in rat liver  and isolated primary hepatocytes [41, 42]. Indeed, stresses such as forced running, immobilization and exposure to cold increase rat liver TDO activity . Taken together, our findings raise the possibility that TDO may in part contribute to the modulation of mood and anxiety-related behavior by stress and environment (see additional file 1, Hypothetical model in Figure S3).
In summary, we provide the first evidence that TDO plays an essential role in the homeostasis of systemic and brain Trp metabolism, including the dominant regulation of serotonergic pathway, under the physiological conditions. TDO also play a role in the maintenance of brain morphology via regulating adult neurogenesis in the hippocampus and subventricular zone. Furthermore, TDO modulates anxiety-related behavior, indicating a role of TDO in higher brain functions. Collectively, the present findings in Tdo-/- mice indicate a direct molecular link between tryptophan metabolism and mental status. Tdo-/- mice will likely prove useful in clarifying the physiological role of Trp metabolism in normal brain function and in psychiatric disorders, and for development of new approaches for therapeutic interventions of mental disorders.
Mice were housed in groups of 3–4 per cage in a room with controlled light (12 h light/dark cycle; lights on at 9 A.M.), humidity, and temperature, and allowed ad libitum access to food and water. Only males were used for the analyses. The acquisition, care, housing, use, and disposition of the animals were in compliance with the institutional laws and regulations of the Osaka University Graduate School of Medicine. All efforts were made to minimize animal discomfort and the number of animals used.
Construction of the targeting vector
Genomic DNA clones of the tdo locus were obtained from the 129/SvJ mouse-derived genomic library (; the kind gift of Dr. T. Morita, Osaka University) using rat tdo cDNA  as a probe. Among the clones, a 12.5-kb tdo genomic fragment containing exons 1 to 3 was used to construct a tdo targeting vector, which was prepared by replacing exons 1 and 2 of the tdo fragment containing the translation initiation site with the PGK-neomycin (Neo) cassette at Hind III-Xba I sites. Subsequently, the Apa I-Eco RI 9.0-kb fragment (left arm, 6.5-kb; right arm, 2.5-kb) was excised and inserted into the MC1 promoter driven diphtheria toxin (DT)-A cassette in order to connect the 5' end of the insert to DT-A.
Disruption of the tdo locus
R1 embryonic stem (ES) cells (kindly provided by Dr. A. Nagy, Mt. Sinai Hospital, Canada, via Dr. H. Kondo, Osaka University, Japan) were electroporated with linearized targeting vector DNA, and selected with G418. G418-resistant ES clones harboring the desired homologous recombinations were verified by Southern blot analysis as previously described [44, 45] after the genomic DNA was digested with Pvu II, using a probe specific for the intron sequence between exons 3 and 4 of tdo (Figure 1B). ES cells that underwent homologous recombination were microinjected into(C57BL/6 × DBA/2) F1 (BDF1) blastocytes. Male chimeras were crossed with C57BL/6 females to generate germ-line heterozygous offspring, with transmission of the targeted allele verified by Southern blot analysis. After backcrossing with wild-type C57BL/6 mice (SLC, Shizuoka, Japan) for 5 generations, homozygous tdo mutants and wild-type animals were obtained by intercrossing heterozygotes. Genotyping of progeny was performed by Southern blot analysis of tail-derived genomic DNA.
RNA purification and quantitative real-time RT-PCR
Total RNA was purified from the livers of 15-week-old wild-type (Tdo+/+), heterozygote (Tdo+/-), and homozygote (Tdo-/-) mice using a TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Quantitative real-time RT-PCR was carried out and mRNA levels were calculated as described previously . For amplification of mouse tdo, tph2, and gapdh (the endogenous control), Universal PCR master mix and FAM dye-labeled Taq-Man MGB probes (Applied Biosystems) were used for mouse tdo (exons 4 and 5, Mm00431715), tryptophan hydroxylase-2 (tph2, Mm00557717_m1) and for rodent gapdh (Taq-Man rodent GAPDH control reagents VIC probe), and the results expressed as the mean ± S.E.
Western blot analyses of liver lysates from 15-week-old Tdo-/-, Tdo+/-, and Tdo+/+ mice were done using anti-rat TDO antiserum (1:2,000, ).
Assay for TDO activity
Liver homogenates were obtained from 10-week-old Tdo-/-, Tdo+/-, and Tdo+/+ mice. Assays for hepatic TDO activity were carried out using L-Trp as substrate as previously described , with activity expressed as μmol of kynurenine formed per hour per gram of wet liver weight.
Measurement of amino acids in plasma and brain
Plasma was deproteinated with 5% sulfosalicylic acid, filtered, and immediately analyzed for amino acid concentrations using automated ion-exchange chromatography with lithium-based buffers on a high-speed amino acid analyzer (L8500, Hitachi, Japan), or stored at -80°C. Micro-dissected brain tissues were rapidly removed after perfusion with ice-cold Hanks' balanced salt solution, and extracted in a solution containing 0.5 M HClO4 and 0.025% EDTA. The tissue extracts were incubated on ice and centrifuged at 12,000 g at 4°C. The collected supernatants were filtered and amino acid concentrations were determined using the amino acid analyzer (Hitachi).
Quantitation of Trp metabolites
Plasma samples treated with trichloroacetic acid (TCA) were prepared in the same way as that used for amino acid analysis. Plasma levels of Trp metabolites (Trp, Kyn, KYNA, ILA, IAA, 5-HT, and 5-HIAA) were determined using HPLC-FD and HPLC-UV systems as previously described . The column used for HPLC was a reversed-phase C18 (2.0 × 250 mm hypersil BDS column, Hewlett Packard). The Agilent 1100 series fluorescence detector utilized the following excitation and emission wavelengths: 287 nm/340 nm for Trp, ILA, IAA, 5-HT, and 5-HIAA, and 254 nm/404 nm for KYNA. UV signals for Kyn were monitored at 365 nm.
Brain samples were prepared in the same way as those for amino acid analysis. Trp, 5-HT, and 5-HIAA were quantified as previously described , with fluorescence detector signals monitored at excitation and emission wavelengths 287 nm/340 nm.
Assay for TPH activity
TPH activity in fresh brainstem from 15-week-old mice of each genotype was determined as previously described  with slight modifications. Assay mixture containing 0.3 mM L-Trp, 0.1 mM Fe(NH4)2(SO4)2, 1 mM 6-methyl-tetrahydropterin (6-MPH4), 2 mM NSD1015 (a inhibitor of the aromatic amino acid decarboxylase), 25 mM DTT, 2 mg/ml catalase, and 50 mM HEPES, pH 7.2 were reacted at 30°C for 20 min with shaking in the dark. The reaction was terminated by the addition of saturated TCA to a final concentration of 10%, and the mixture was then chilled on ice and centrifuged at 4°C. 5-hydroxytryptophan (5-HTP) in the supernatant was separated on a μBondapak ODS C18 HPLC column (Waters) with a mobile phase of 2.5% methanol in 40 mM sodium acetate buffer, pH 3.5. A F1050 fluorescence detector (Hitachi) detected signals at the excitation and emission wavelengths of 287 and 340 nm, respectively. TPH enzymatic activity was normalized to total protein in the homogenate and expressed as nmol 5-HTP/mg/h at 30°C.
Histological and immunohistochemical analyses
Mice were deeply anesthetized and transcardially perfused with ice-cold phosphate-buffered saline (PBS; pH 7.4), followed by 4% paraformaldehyde (PFA) in PBS. For analyses of brain morphology and PSA-NCAM immunostaining, the brains were removed and embedded in paraffin. Serial coronal sections (5 μm) were prepared, deparaffinized and stained with hematoxylin and eosin (H&E), or used for PSA-NCAM immunostaining. For most immunostaining, brains were removed and cryoprotected in 10% and 20% sucrose in PBS at 4°C after perfusion with 4% PFA in PBS. Twenty-micrometer sections were prepared using a cryostat, mounted on APS -coated slides, and stored at -80°C. Primary antibodies were applied to the sections for 24 or 48 h at 4°C after incubation with blocking buffer containing 10% goat or donkey serum and 0.3% Triton X-100 in PBS. The following primary antibodies were used: Ki67 (DAKO), nestin (BD Pharmingen), GFAP (Chemicon), DCX (Santa Cruz), NeuN (Chemicon), βIII-tubulin (TuJ1; Covance Research), and PSA-NCAM (AbCys S.A.).
After washing with PBS, either Alexa Fluor 488- or Alexa Fluor 546-conjugated secondary antibody and the nuclear counterstaining reagent TO-PRO-3 iodide (Molecular Probes) in PBS were applied. Sections were then washed with PBS, mounted in Crystal mount (Biomeda) and observed under a LSM 510 PASCAL confocal microscope (Zeiss).
Quantification of ventricular size
The size of each ventricle (lateral ventricle, between 1.1 and 0.86 mm rostral; dorsal 3rd ventricle, between 1.7 and 1.94 mm caudal; and aqueduct, 4.36 and 4.48 mm caudal to the bregma, as per Franklin and Paxinos, ) was analyzed in six paraffin-embedded sections per animal (n = 4 per group) and quantified using WinRoof software (Mitani Corp., Japan), with the average size of the ventricles in Tdo+/+ mice defined as 100%. Results are expressed as the mean ± S.E.
Administration of BrdU and staining
BrdU (5-bromo-2'-deoxyuridine) injections and subsequent analyses were performed  using a BrdU-specific antibody (Oxford Laboratory). In brief, to assess dividing progenitors, 13-week-old mice were administered BrdU (4 × 75 mg/kg) by intraperitoneal injection at 2 h intervals and sacrificed 24 h after the last injection. To determine the fate of BrdU-labeled cells, 9-week-old mice were administered BrdU (4 × 75 mg/kg) and sacrificed 28 days after the last injection. Brain sections were prepared as described above. For BrdU immunohistochemistry, sections were fixed in acetone, treated with 1 N HCl for 30 min at 60°C to denature DNA, and rinsed in PBS. Subsequent processes for immunolabeling with the BrdU antibody were identical to those described above.
Cell quantitation in the hippocampus
Hippocampal sections from 13-week-old Tdo+/+ and Tdo-/- mice prepared 1 and 28 days after the injection of BrdU were examined for the number of cells immunopositive for NeuN, TuJ1, PSA-NCAM, DCX, nestin, GFAP, BrdU, and/or Ki67 . In these experiments, cells in the total surface of the granular cell layer (GCL) or in the subgranular zone (SGZ) in the dentate gyrus (DG) were counted in one of at least five sections per animal (n > 3 per group) between 1.7 and 2.06 mm caudal to the bregma, as per Franklin and Paxinos . The total number of nuclei/slice in the SGZ and GCL was defined as total cell number. The percentage of double-immunostained cells was obtained by analyzing three-dimensional reconstructed BrdU-positive nuclei in x-z and y-z orthogonal projections for the presence of cell markers.
Behavioral assessment using the elevated plus maze test and open field test
All behavioral tests (elevated plus maze and open field test) were conducted between 9:30 AM and 1:00 PM. All experiments were monitored by an automated video camera system and analyzed with Ethovision Ver. 2.3.19 software (Noldus, Wageningen, Netherlands).
Elevated plus maze
Tdo+/+ and Tdo-/- mice (13 to 15 weeks old) were tested in an elevated plus maze according to Lister et al.  with slight modification. In brief, the plus maze consisted of two open (30 × 6 cm) and two wall-enclosed arms (30 × 6 × 15 cm) connected by a central platform (6 × 6 cm). The apparatus was elevated 40 cm above the floor. The mouse was placed in the central zone facing an open arm, which the animal would usually enter first, and exploratory behavior during a 5 min test period was monitored. Testing took place during the light phase under standard light.
Open field test
Open field tests were conducted in 13- to 15-week-old Tdo+/+ and Tdo-/- mice according to the method of Paylor et al. . Briefly, the open field consisted of four adjacent activity chambers (40 × 40 × 40 cm) surrounded by walls, with the field lit from above. Mice were released into the center of the field and allowed to roam the open field for 30 min. Total distance moved, time spent and the distances moved in both the margins (≤10 cm of the walls) and center zone of the field (>10 cm from walls) were measured. The ratio of the distance moved in the center to the total distance moved was calculated and used as a measure of anxiety-related behavior.
Statistical analysis was carried out using StatView software version 5.0.1 (SAS Institute, Cary, NC). Student's t-test was used for the amino acid analyses, while the other data were analyzed by one-way factorial analysis of variance (ANOVA). A post-hoc test was carried out for ANOVA p-values less than 0.05. Statistical significance was determined using Scheffe's post-hoc test at p < 0.05. Statistical results were indicated as a p-value by the post-hoc test.
5-hydroxytryptamine or serotonin
essential amino acids
large neutral amino acids
elevated plus maze test
open field test.
We are grateful to Dr. Y. Uno of the Supply Center of Inbred Animals of Osaka Univ. for his help and advice with the microinjection of targeted clones into blastocysts and to Mr. W. Furutani in the Osaka Univ. for help with the amino acid analyzer. This work was supported in part by research grants from COE to T.N., and by grants from the Ministry of Education, Science, Technology, Sports and Culture and the Ministry of Health and Welfare of Japan to T.N. and H.F.
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