Figure 1

Sampling region for evaluating the effect of HFS on neurogenesis in the DG. (A) Anatomical organization of the entorhinal-hippocampal dentate gyrus (DG) pathway. Abbreviations: Rec, recording electrode; Stim, stimulating electrode; MPP, medial perforant path fibers (orange); LPP, lateral perforant path fibers (green). (B) Overhead schematic view of a rat brain. The orange area indicates the sampling area (-3.0 mm to -5.0 mm relative to bregma) for the assay to evaluate neurogenesis. HPC, Hippocampus. (C) PS amplitudes of the DG at 0 day and 1 day and 7 days from start fEPSP recording (no HFS, test pulse only). (D, E) PS amplitudes of the DG at pre-HFS, and 1 day and 7 days after the HFS treatment in HFS (90) (D) and HFS (500) (E) groups. Typical fEPSP traces evoked before HFS (left) and 1 day after HFS (middle) and 7 days after HFS (right) are also shown. Error bars indicate SEM. The number of animals used is indicated by "n". (F) Nuclear staining by DAPI (upper photos), vesl-1S mRNA by in situ hybridization (middle photos), and F-actin staining by phalloidin (lower photos) through the anterior-posterior extent of hippocampus of HFS (500)-treated rat. (a-f) indicate the positions in the hippocampus from the anterior to posterior, and correspond to (a-f) in Fig 1B. Scale bars, 500 μm.