Figure 2
From: Neuronal and microglial mechanisms of neuropathic pain
Simultaneous confocal imaging and patch clamp recordings in microglia in situ. ATP-containing pipette induced both outward current and chemotaxis in brain microglia. Outward current appears after pipette approaches, while disappears after the pipette moves away from the recording microglia (middle). DIC image showed the recording pipette and ATP-containing pipette. Fluorescent images showed that the chemotactic effect during the insertion of the ATP pipette (upper). When the pipette was removed out of the slice, the moving terminals rapidly disappeared (lower, right). Scale bar, 15 μm. Modified from [53].