Figure 6

Spinal Toll-like receptor (TLR4) is involved in bone cancer pain. (A) showing the changes of TLR4 mRNA levels in bilateral dorsal horn of spinal cord 8 and 16 days after intra-tibial injection of PBS, Heat-killed or live Walker 256 cells. (B) Showing the blocking effect of single intrathecal administration of naloxone (60 μg) on elevated spinal IL-1β and TNF-α mRNA expression induced by intra-tibial inoculation of Walker 256 cells. (C) Anti-allodynic effects induced by single intrathecal administration of naloxone (60 μg). On the day 8 after Walker 256 inoculation, naloxone (60 μg), a nonsteroselective TLR4 antagonist, significantly increased the mechanical thresholds to von Frey hair stimulation 45, 135 mins after i.t. treatment. &&p < 0.01 vs. normal rats; *p < 0.05, **p < 0.01 vs. Walker 256 inoculated rats; #p < 0.05, ##p < 0.01 vs. normal saline treatment.