Figure 4
From: Activation of GABAB receptors inhibits protein kinase B /Glycogen Synthase Kinase 3 signaling
Activation of GABA B receptors elevates GSK-3α/β phosphorylation in rat hippocampal slices, and this effect can not be blocked by PTX when pretreating primary cultured hippocampal neurons. A. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) levels in extract prepared from rat hippocampal slices in the presence of GABAB receptor agonist and/or antagonist. GSK-3α/β was used as a loading control. B. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). C. Western blot analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9) levels in extract prepared from rat primary cultured hippocampal neurons pretreated with PTX (200 ng/ml, 14–18 h) in the presence or absence of GABAB receptor agonist SKF97541. GSK-3α/β was used as a loading control. D. Densitometric analysis of phosphorylated GSK-3α/β (Ser-21/Ser-9). The intensity of phospho-GSK-3α/β was quantified by densitometry (software: Image J, NIH). Data were analyzed by one-way or two-way ANOVA (*P < 0.05, **P < 0.01,***P < 0.001, n = 3).