Table 1 Primers for quantification of expression of GABA A R subunit genes in granule cells with real-time PCR
Gene | NCBI mRNA reference sequence(s) | Primers (5′-3′) | Position | Amplicon length (bp) |
|---|---|---|---|---|
Gabra1 | NM_010250.4 | F: GAGCAGAAGTTGTCTATGAGTGG | 1349, exon 7 | 164 |
R: GTGGAAGTGAGTCGTCATAACC | 1512, exon 8 | |||
Gabra6 | NM_001099641.1 | F: CTACTCTGAAAATGTCAGTCGGATTC | 364, exon 2 | 123 |
(variant 1) | R: CCAAAGCTGGTCACATAGATGTCT | 486, exon 3 | ||
NM_008068.2 | 364, exon 2 | 123 | ||
(variant 2) | 486, exon 3 | |||
Gabrb2 | NM_008070.3 | F: AGGGGCTACTTTGGGATTTGG | 446, exon 2 | 108 |
R: TCTGTCCACCGTCTCTTTAACC | 553, exon 3 | |||
Gabrb3 | NM_008071.3 | F: TTGCGGAGAAGACAGCCAAG | 1128, exon 9 | 100 |
(variant 1) | R: TGAACATCCATCGGTGCTAATAGG | 1227, exon 10 | ||
NM_001038701.1 | 1060, exon 9 | 100 | ||
(variant 2) | 1159, exon 10 | |||
Gabrd | NM_008072.2 | F: GCCAGCATTGACCATATCTCAG | 324, exon 3 | 169 |
R: CATTCACGATGAAGGTGTCAGG | 492, exon 4 | |||
Gabrg2 | NM_008073.2 | F: CTTACATTCCCTGCACACTCATC | 1181, exon 7 | 140 |
(variant 1) | R: AGATTTTCTGGCTATGGTGCTT | 1320, exon 8 | ||
NM_177408.5 | 822, exon 7 | 140 | ||
(variant 2) | 961, exon 8 |