Figure 3
From: Zinc transporter-1 concentrates at the postsynaptic density of hippocampal synapses
Co-labeling of ZnT1 and vesicular Zn2+. (A) Sections of CA1 synapses stained for vesicular Zn2+ (solid arrows) and ZnT1 (solid arrowheads, synaptic; white arrowheads, extrasynaptic). (B) ZN- synapses labeled for ZnT1 at the synaptic junction. Right, Consecutive sections of a ZN- axospinous synapse labeled for ZnT1. b, bouton; s, spine. Scale bars, 0.2 μm. (C) Surface density of gold particles showing the accumulation of ZnT1 in synaptic junctions (i.e. ZN + and ZN- synapses pooled) compared with various extrasynaptic membrane domains. (D) Sequence analysis of ZnT members showing that only ZnT1 contains a canonical PDZ type I motif in its C-terminus.