Figure 5
p-mTOR activation in APPswe/PS1ΔE9 mice. Shown is immunohistochemical staining (A) for p-mTOR (pSer2481) staining in representative fields of coronal tissue from wild type (wt), APPswe/PS1ΔE9 (APPswe), mGluR5-/- and APPswe/mGluR5-/- mice at 12 months of age (B) and mTOR (pSer2481) staining in representative fields of coronal tissue from wt, APPswe, mGluR5-/-, and APPswe/mGluR5-/- mice at 12 months of age. Scale bar = 100 μm. (C) The graph shows the number of p-mTOR positive cells counted 8100 μm2 regions of interests in the cortex of 12 month old from wt, APPswe, mGluR5-/-, and APPswe/mGluR5-/- mice. Data represents the mean ± SEM of four independent experiments. The * indicates statistically significant (p < 0.05) differences between wt and APPswe mice. (D) The graph shows the number of total mTOR positive cells counted 8100 μm2 regions of interests in the cortex of 12 month old wt, APPswe, mGluR5-/-, and APPswe/mGluR5-/-. Data represents the mean ± SEM of four independent experiments. Shown in (E) is a representative immunoblot for p-mTOR (pSer2481), total mTOR and actin in lysates derived from cortical brain section from wt and APPswe mice that either express mGluR5 (+/+) or do not express mGluR5 (-/-). (F) Shown in the graph is the analysis of the mean ± SD for four independent experiments for mTOR phosphorylation in wt and APPswe mouse cellular lysates that either express mGluR5 or do not express mGluR5. The * indicates statistically significant (P < 0.05) differences between mTOR phosphorylations in APPswe as compared to wt mice.