Figure 3
From: Inorganic polyphosphate regulates neuronal excitability through modulation of voltage-gated channels
PolyP significantly inhibits I KV and activates I CaV . A) Representative IKV traces recorded during a ramp protocol with 0.3 μM TTX in the bath (see Methods). At +24 mV, IKV decreased by 39.5% with polyP application. B) Current densities before and after polyP application. 0.3 μM TTX was in the bath. In EBS, IKV decreased by 36.3% ± 5.4 with polyP application (n = 12). With 500 μM suramin in the bath, IKV decreased by 34.5% ± 6.3 with polyP application (n = 6). Using a Cs-gluconate internal solution, Ioutward increased by 1.7% ± 0.3 with polyP application (n = 6). C) Occasionally, ICaV was evoked by polyP application (4 of 7 cells). Shown here are representative current traces where polyP application activated ICaV at -58 mV and blocked IKV (at +24 mV) by 41.8%.