Figure 2
From: Histamine impairs midbrain dopaminergic development in vivo by activating histamine type 1 receptors
Histaminergic receptors are expressed in rat NSPC ventral midbrain cultures. (A) RT-PCR experiments to detect H1, H2 and H3 receptor mRNAs in proliferating (Prolif) and differentiated (Diff) cultured cells from E12 VM. One μg of total RNA was used for reverse transcription. The expression of gene transcripts for H1, H2 and H3 receptors were analyzed by electrophoresis on a 2% agarose gel. Rat adult cerebral cortex (Ctx) was used as positive control and RNA without retrotranscriptase (−RT) as negative control. (B) Western blot experiments performed to detect H1 and H2 receptors in E12 midbrain proliferating and differentiated cells. Rat adult cerebral cortex (Ctx) was used as positive control. Thirty μg of protein were resolved on 8% SDS-PAGE, transferred to nitrocellulose membranes, and probed with anti-HA receptors antibodies. Immunoreactive bands were detected by enhanced chemiluminescence. In RT-PCR and Western blot experiments, GAPDH was used as control.