Fig. 1

Activation of Wnt5a-Ca²⁺ signaling in radially migrating cortical neurons. a Protocol for Fluo-4 Ca²⁺calcium imaging in immature migrating cortical neurons. b Top, image acquired from a Fluo-4-labeled acute slice, in which immature migrating neurons expressing TagRFP where identified in CP and IZ. Bottom, magnified image of an immature cortical neuron in CP from the boxed region in the upper panels. Scale bars: 50 μm (top) and 10 μm (bottom). c Left, Fluo-4 Ca²⁺ imaging showing immature migrating cortical neurons experiencing spontaneous Ca²⁺ activity. The neurons are magnified from the boxed areas in (b). Right, Ca²⁺ transient traces of immature cortical neurons, with vertical lines corresponding to the time points of the left images. Scale bars: 10 μm. d Wnt5a administration evoked Ca²⁺ transients in immature migrating neurons, both in CP (#1–4 cells) and IZ (#5, #6 cells). Several of the immature neurons in CP (e.g. #1–3 cells) showed sustained Ca²⁺ oscillations. Scale bar: 50 μm