Fig. 2
From: Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization
Axon-specific morphogenetic effects of Wnt5a in immature cultured cortical neurons. a Left, cortical neurons were stimulated with Wnt5a from 6 h until 48 h after plating. Wnt5a administration induced elongation of axons (arrowheads) but not dendrites (arrows). Right, a scatter plot of data points for both axonal and dendritic lengths obtained from individual neurons. Number of neurons: n = 15 for both Vehicle and Wnt5a groups; Scale bar: 50 μm. b Quantification of morphometric parameters in Wnt5a-stimulated neurons. For quantification, the total length, the branch tip number, and the longest processes were calculated over the axonal or the dendritic arborization for all branches exceeding 7 μm in length. Top: quantification of Wnt5a-stimulated neurons. The total length of axons and the length of the longest axons were increased. Bottom: none of the 3 morphometric parameters were significantly altered in dendrites. Number of neurons: n = 15 for both the vehicle and Wnt5a groups. **p < 0.01, n.s., not significant (p > 0.05) (t-test)