Fig. 4
From: Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIα pathway during neuronal polarization
CaMKIα mediates Wnt5a-facilitated axon outgrowth. a Left, Specific effects of Wnt5a treatment and CaMKIα knockdown on total axonal lengths during neuronal polarization. Wnt5a treatment facilitated axon outgrowth and CaMKIα KD abolished this facilitation, in keeping with results from CaMKK blockade. Note that CaMKIα KD alone inhibited basal axonal elongation even in the absence of Wnt5a. Right, Neither Wnt5a treatment nor CaMKIα KD had any detectable effect on total dendritic lengths. Number of neurons: n = 15 for all groups. ***p < 0.001, **p < 0.01, n.s., not significant (p > 0.05) (two-way ANOVA with Turkey’s test). b Suppression of Wnt5a-stimulated axonal elongation by CaMKIα KD is rescued by co-expression of a shRNA-resistant wild-type CaMKIα (WTres). Number of neurons: n = 15 for all groups. ***p < 0.001 (one-way ANOVA with Turkey’s test)