Fig. 3

Anterior-shifted Wg expression results in a reduced PD-Angle. a-b The PD-Angle is significantly decreased in TS and wg ^spd-fg in both late embryos and third instar larvae. (n =26- 57). c-d wg expression region is significantly wider in both wild type TS2-3 and wg ^spd-fg AS4-6, when compared to wild type AS4-6. The distance between the center of wg signal and posterior segmental boundary is also significantly increased when normalized to segment width. (n = 20–29). e-f Wg expression is markedly increased in TS2-3 of wild-type (w ¹¹¹⁸) and in AS4-6 of wg ^spd-fg mutants, and the center of Wg signal is also significantly anteriorly shifted. (n = 23-36). g Schematic diagram of Wg expression pattern and dendrite orientation. The ddaE neuron is located at the anterior side and adjacent to Wg-expressing cells, and the two primary dendrites are orientated towards the posterior when Wg expression is reduced (as in wg ^l-12 and wg-Gal4 > wg ^RNAi), or when Wg is ectopically expressed in the anterior region (as in wg ^spd-fg and wild-type TS). Scale bar: 50 μm in (a) and 10 μm in (c and e)