Fig. 6

Dishevelled and Racl are involved in regulating dendrite orientation. a-b Knockdown of dsh results in a significant reduction in the PD-Angle, which is rescued by expressing Dsh in the neuron. (n = 24–46). c-d The PD-Angle is decreased when Rac1 is suppressed by expressing either rac1 ^RNAi or Rac1^T17N (a dominant negative form), whereas overexpressing Rac1 shows no effect on the PD-Angle. (n = 38–53). e-f Knockdown of rac1 partially rescues the reduced PD-Angle in wg ^spd-fg mutant background. (n = 59–77). g-h Knockdown rac1 does not further decrease the PD-Angle in wg ^l-12 mutant background. (n = 24–36). i-j Knockdown of rac1 does not lead to further change of the PD-Angle in either fmi mutant stan ^f00907 or fz Knockdown of background. (n = 37–76). k Schematic diagram of molecules in Wg signal pathway that regulates dendrite orientation. Fz, Fmi, Dsh, and Rac1 are essential for mediating Wg signal to regulate directional growth of dendrites, while G proteins, β-catenin pathway, and Cdc42/Rho1 are dispensable for the dendrite directional growth of ddaE neuron. Red arrows indicate the initial parts of primary dendrites. Scale bar: 50 μm