Fig. 13

AP-2δ expression and apoptosis in the superior colliculus. (a) Wild-type P1 mouse brain sagittal section immunostained with anti-AP-2δ antibody. Photographs were taken with a 5× lens and photo-merged in Photoshop. A positive signal is observed in the inferior colliculus (IC), superior colliculus (SC), pretectum and anterior olfactory nucleus (AON). b Sagittal section from wild-type P1 brain co-immunostained with anti-AP-2δ and anti-Brn3c antibodies followed by secondary antibodies conjugated with Alexa 488 and Alexa 555, respectively. AP-2δ-positive cells are observed throughout the SC, with Brn3c cells co-expressed with AP-2δ (e.g. see arrows) in a subset of AP-2δ-positive cells. c Sagittal section from AP-2δ−/− P1 brain immunostained with anti-caspase 3 antibody showing apoptosis in the inferior colliculus and to a much lesser extent in the SC. Inset is magnified on the right. Arrows point to apoptotic cells