Fig. 1

Changes in adenosine receptor expression in vivo and in vitro. a Western blot analysis of adenosine receptor expression at different time points following ICH in vivo. A1AR levels peaked at 48 h. b Quantification of the results in panel A. Bars represent relative protein levels. The mean values of the protein levels in the sham group were normalized to 1.0. *p < 0.05 for the 48 h ICH group versus the sham group. c Western blots of adenosine receptor expression in vitro. A1AR levels peaked at 48 h. d Quantification of the results in panel C. Bars represents relative protein levels. The mean values of the protein levels in the control group were normalized to 1.0. *p < 0.05 for the 48 h OxyHb group versus the control group. e and f Immunofluorescence in vivo. Double immunofluorescence analysis was performed with A1AR antibodies (green) and neuronal or astrocyte marker (NeuN/GFAP, red), and nuclei were fluorescently labeled with DAPI (blue). Scale bar = 32 μm. G: Immunofluorescence in vitro. Double immunofluorescence analysis was performed with A1AR antibodies (green) and neuronal marker (NeuN, red), and nuclei were fluorescently labeled with DAPI (blue). Scale bar = 20 μm