Fig. 2

Microinjection of CaCl₂ (25 or 50 nmol) into the dorsal raphe nucleus (DRN) increased monoamine neurotransmitters. Norepinephrine (NE), serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) levels in the dorsal raphe nucleus (a), prefrontal cortex (b), hypothalamus (c), and locus coeruleus (d) were analyzed. The relationship between the 5-HT in the DRN and 5-HT in the hypothalamus (e) or NE in the LC (f) were analyzed (n = 6 ~ 8/group). Data are calculated as ng/g protein and expressed as mean ± SEM, *p < 0.05 and **p < 0.01 vs Vehicle (Student-Newman-Keuls test and Pearson’s correlation analysis)