Fig. 3

Acute impairment of mitochondrial transport in axons versus dendrites. a Representative images of a DIV6 hippocampal neuron expressing Mito-DsRed (Mito, left panel) and Synaptophysin-YFP (Syn, middle panel). Both images were used to identify the axonal and dendritic compartments of the neuron, as highlighted by the schematic drawing shown in the right panel. Here, solid lines represent the dendrites and dashed lines represent the axon. Scale bars: 20 μm. b and c Bar graphs showing the quantitative results on the acute impairment of mitochondrial transport in number (b) and in speed (c) by Aβ–O in axons and dendrites. Numbers indicate the number of neurons analyzed in each group (from at least three batches of culture). Error bars indicate SD (b) and SEM (c). *p < 0.05 and **p < 0.005 in comparison to the correspondent control or between axons and dendrites (Student’s t-test)