Fig. 4

Effects of Aβ-O treatment and removal on β-catenin in primary cortical neurons. a Primary neurons grown on coverslips were treated as in Fig. 1, followed by immunofluorescence staining with an anti-β-catenin antibody. Bottom panel shows high-magnification images of the regions depicted by white squares in upper images. Scale bar = 20 μm. b Immunofluorescence intensity of β-catenin staining was quantified as described in Methods and expressed relative to levels in control neurons on day 2 or 4. Data represent means ± SEM from three separate experiments. c Primary neurons were treated as in Fig. 1, followed by Western blot analysis of cell lysates using the indicated antibodies. d, e β-catenin (d) levels and p-β-catenin/total β-catenin ratios (e) expressed relative to those in control neurons on day 2. Data represent means ± SEM from three separate experiments. *p < 0.05, **p < 0.01, compared with control. #p < 0.05, ##p < 0.01, compared with Aβ-O-treated cells