Fig. 2

Normal myelination in Polr3a KI/KI and KI/KO mice. a-b) Luxol Fast Blue staining of coronal sections (a) showing the corpus callosum (long arrow) and dorsal fornix (short arrow), both myelinated, and of sagittal sections (b) of the cerebellum. Staining was performed on three 90 days old mice per group and representative images are shown for each group. Scale bar = 100 μm. c) Immunoblots of myelin proteins using total protein extracts from the brain of 90 days old WT, KI/KI and KI/KO mice