Fig. 4

SorLA and PICK1 co-localize. A SY5Y cells were stained with anti-PICK1 (green) and anti-SorLA (magenta) antibodies. Magnifications of two selected areas (boxes) are shown. B Immunocytochemical localization of IL2R-SorLA and tdTomato-PICK1 in transfected dissociated primary cultured hippocampal neurons using anti-IL2R (green) and anti-tdTomato (magenta) antibodies. Magnification of a selected area (boxes) are shown. C Immunolocalization of endogenous SorLA and PICK1 in dissociated primary cultured hippocampal neurons using anti-SorLA (green) and anti-PICK1 (magenta) antibodies. Magnification of a selected area (boxes) are shown. D Respective Manders’ coefficient of SorLA and PICK1 using antibodies (AB) against the endogenous proteins or of overexpressed (OE) IL2R-SorLA and tdTomato-PICK1. E Primary sequence of the SorLA cytoplasmic domain. Amino acid motifs mediating interaction with retromer, AP-1 and AP-2 and GGA1 and GGA2 are indicated in grey. The here described PICK1 interaction motif is indicated in blue. Scale bars: 10 µm