Fig. 3

Effects of activation/inhibition of IAPP receptor. CLS after stimulation with vehicle (A), IAPP (B), and IAPP together with the antagonist AC187 (C), scale bar (A–C): 100 µm. Quantification of the proportion of round-shaped HBVP per CLS after stimulation with vehicle, IAPP, and IAPP with AC187 is seen in (D). Untreated CLS (E) vs. after stimulation with the IAPP agonist pramlintide (F), scale bar in (E) and (F): 100 µm. Graph in (G) shows quantification of the proportion of round-shaped HBVP per CLS for untreated and pramlintide stimulation. All images were acquired with the 10 × objective. Experiments in (D) and (G) were performed in 6 replicates. In (D), data was analyzed using one-way ANOVA followed by Dunnets correction test (n = 2 comparisons), in (G), data was analyzed by student t-test. Each point represent the mean ± SD. Significant difference at *p < 0.05