Fig. 1

The neuron activity in the ACC is independent of the histamine-induced scratching response. (A) The schematic image of the miniscope imaging system. DAQ hardware received the calcium signals from miniscope, and behavior was simultaneously recorded by camera. α: miniscope, β: commutator, preventing the twisting of coaxial cable by rotating, γ: behavior camera. (B) The representative image of the ACC after (upper) and during (lower) the miniscope imaging. The region between the two red dash lines is ACC layer 2/3. Some of the dendrites reached toward the ACC layer 5. (C) Calcium transients in representative 3 neurons after histamine injection. (D) The change in mean dF/F did not depend on the scratching response. Each row represents the mean dF/F of one mouse. Red lines represent time points of scratching bouts. (E) Mean calcium level 1 s before and after scratching bouts. Mean intensity was reduced immediate after scratching bouts. Same color represents data come from same animal. (F) The calcium level of each neuron was reduced immediate after scratching bouts (n = 287 neurons of 5 mice; D’Agostino & Pearson omnibus normality test (post-pre), p < 0.0001; Wilcoxon signed rank test, *** p < 0.0001)