Fig. 6
From: A new AAV tool for highly preferentially targeting hippocampal CA2
Efferent contralateral and longitudinal projections of dCA2 infected with AAV9/M1-Cre. A Schematic of unilateral stereotactic injection at dCA2 of WT mouse with AAV9/M1-Cre & AAV9/EF1α-DIO-EGFP (n = 10 mice). B Image showing M1-EGFP axons traversing to the hippocampal commissure. C Image showing M1-EGFP axons traversing to the hippocampal fornix. D, E Fluorescence images showing dCA2 unilaterally injected with M1-EGFP displayed projections to the contralateral dorsolateral CA1, CA2 and CA3 (E). F, G Images showing M1-EGFP axons had projections to ipsilateral CA3 and CA1. H, I Confocal images showing dCA2 bilaterally injected with M1-EGFP displayed efferent projections to septal areas LS (H) and MS (I) (n = 16 mice). J–L Confocal images showing dCA2 bilaterally co-injected with AAV9/M1-Cre & AAV9/hSyn-DIO-mcherry displayed longitudinal projections to vmh (I), sthy (J) and pmv (K) (n = 9 mice). M Coronal section of mouse brain infected with M1-Cre & EF1α-DIO-EGFP (M1-EGFP) in dCA2 (n = 39 mice). Stained with DAPI (blue). Scale bars: 1 mm (M), 500 μm (B, C, F), 200 μm (G, H), 100 μm (D, E, I), 50 μm (J–L)