Fig. 7
From: A new AAV tool for highly preferentially targeting hippocampal CA2
Expression pattern of the chemogenetic inhibitor DIO-hM4Di induced by M1-Cre in dCA2. A Schematics of stereotactic injection of AAV9/M1-Cre & AAV9/EF1α-DIO-hM4Di-mcherry in WT dCA2. B Magnified fluorescence images of RGS14(green), M1-Cre/DIO-hM4Di-mcherry(red) and trio-channels displaying hM4Di expressed within RGS14 stained position. C1–C5 Images showing representative coronal serial sections of dCA2 infected with hM4Di-mcherry (red) from beak to tail (left). Images displaying mcherry located in RGS14 region (right) (n = 21 mice). D the fluorescence image of half-brain slice which CA2 infected with M1-Cre/DIO-hM4Di-mcherry. Scale bars: 1 mm (D), 200 μm (C1–C5), 100 μm (B)