Fig. 3

LTD-IE affects AP shape differently between wild-type and STIM1^PKO. In representative AP traces, AP thresholds are pointed by red (wild-type) or magenta circle (STIM1^PKO), and red arrows indicate FWHM. A FWHM of wild-type through the time. FWHM was significantly increased at 1 h after training compared to sham control (F _{1, 23} = 14.0, p = 0.001). The value of FWHM at the 4 h time point was significantly lower than all other time points (sham vs. 4 h, F _{1, 28} = 4.59, p = 0.041; 1 h vs. 4 h, F _{1, 23} = 17.3, p < 0.001; 4 h vs. 24 h, F _{1, 30} = 4.92, p = 0.034). B Upstroke speed of wild-type through time. Upstroke speed was significantly increased between 1 and 4 h time points (1 h vs. 4 h, F _{1, 23} = 8.49, p < 0.008) C Downstroke speed of wild-type through the time (sham vs. 4 h, F _{1, 28} = 5.82, p = 0.023; 1 h vs. 4 h, F _{1, 23} = 9.27, p = 0.006; 4 h vs. 24 h, F _{1, 30} = 4.36, p = 0.045). D–F STIM1^PKO showed no statistically significant alterations in FWHM, up- and down-stroke speeds. Sample numbers of wild-type (sham n = 15, 1 h n = 10, 4 h n = 15, 24 h n = 17) and STIM1^PKO (sham n = 16, 1 h n = 15, 4 h n = 16, 24 h n = 10) are the same for all panels. Two-way ANOVA was used for all panels. The graphs are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001