Fig. 2
From: The lateral habenula nucleus regulates pruritic sensation and emotion
Chronic itch enhances GluLHb neural excitability. A Schematic diagram of the experimental design. B Representative images of dorsal neck skin of the water only (Control) and acetone-ether-water (AEW)-treated mice on the seventh day. C Timeline of scratch bouts in the control and AEW groups in C57 mice. D Left: Peri-event plots of the average change of Ca2+ fluorescence intensity in the AEW model. Vertical gray dashed line: onset of AEW-evoked scratching; red, GCaMP6s virus channel recording; green, EGFP control virus channel recording; Middle: the heat map of Ca2+ transients aligned to the beginning of scratching in 15 trials from 3 mice (5 trials per mouse). Vertical white dashed line, onset of AEW-evoked scratching. Color scale indicates △F/F; Right: Quantification analysis of area under the curve (AUC) during 0–5 s of average Ca2+ fluorescence induced by AEW-evoked scratching stimulus in the GCaMP6s group compared with the EGFP group. E, F Representative a trace (E) and summary data for the firing rates (F) of GluLHb neurons recorded from AEW or control mice. (Control, n = 21 cells; AEW, n = 24 cells). *p < 0.05, ***p < 0.001, ****p < 0.0001, not significant (ns). Two-way ANOVA with Bonferroni’s correction for multiple comparisons in (C, F) and two-tailed unpaired Student’s t-test in (D). Data were shown as mean ± SEM