Fig. 4
From: The lateral habenula nucleus regulates pruritic sensation and emotion
GluLHb neural inhibition blocks the itch-associated aversiveness. A An outline of the experimental procedure for chloroquine-induced conditioned place aversion (CPA) test. B Viral targeting of AAV-CaMKIIα-hM4Di-mCherry or AAV-CaMKIIα-mCherry bilaterally into the LHb of C57 mice. C Effect of GluLHb neural inhibition on the acute itch-associated CPA. (hM4Di group, n = 8 mice; mCherry group, n = 8 mice). D An outline of the experimental procedure for chronic itch-induced CPA test. E Effect of GluLHb neural inhibition on the AEW group-associated CPA. (hM4Di group, n = 5 mice; mCherry group, n = 5 mice). F Effect of GluLHb neural inhibition on the AEW control group-associated CPA. (hM4Di group, n = 5 mice; mCherry group, n = 5 mice). Significance was assessed by two-tailed paired Student’s t-test in (C, E) and Wilcoxon signed-rank test for paired test in (F), **p < 0.01, not significant (ns). All data were shown as mean ± SEM