Fig. 1

Effects of FTY720 on behavioral changes in short-term cuprizone-exposed mice. A, B Effects of FTY720 on locomotor activity in mice after methamphetamine administration. Mice were placed in a circular open field for 30 min to measure baseline locomotion, whereafter an intraperitoneal injection of methamphetamine (2 mg/kg) was administered to the mice and locomotion was measured for 60 min. A The locomotion (ambulatory counts) for 5-min bin within the total 90 min session (− 30 to 0 min before and 0 to 60 min after methamphetamine injection). Arrows indicate methamphetamine injection time. B Total locomotion (cumulative horizontal ambulatory counts) after injection of methamphetamine (0-60 min). n = 10–11 per group. C Rotarod measurement of basal locomotor function. The latency to fall from the rotarod (s) with maximum latency set at 120 s (n = 5 per group). All values are Mean ± SEM. Tukey test *p < 0.05, ***p < 0.001. A, C 3way RM ANOVA. B 2way ANOVA ^#p < 0.05, ^###p < 0.001, ^####p < 0.0001