Fig. 2From: C1ql1-Bai3 signaling is necessary for climbing fiber synapse formation in mature Purkinje cells in coordination with neuronal activityIncreased C1ql1 levels in CFs allow adult PCs to be innervated by multiple CFsA Experimental scheme for recording CF-EPSCs. B Representative CF-EPSC traces from wild-type PCs. The blue bar indicates the timing of light stimulation. Increasing the light intensity elicited a single EPSC in control slices (B1) in an all-or-none manner, but multiple EPSCs with a slower rise time in slices overexpressing C1ql1 (B2). C Total CF-EPSC amplitude. The graph shows the sum of the peak amplitudes of single CF-EPSCs or multiple CF-EPSCs. p = 0.0372, two-tailed Welch’s t-test; n = 28 cells from 4 mice (CTRL); n = 29 cells from 5 mice (+ C1ql1). D The percentage of the number of CFs innervating single PCs. The number of EPSCs evoked by distinct CF activation thresholds (number of steps) is shown. p = 0.0234, Mann–Whitney U test; n = 51 cells from 4 mice (CTRL); n = 49 cells from 5 mice (+ C1ql1). E Average of the 10–90% rise time of CF-EPSCs. p = 0.0028, two-tailed Welch’s t-test; n = 28 responses from 4 mice (CTRL); n = 46 responses from 5 mice (+ C1ql1). F Representative CF-EPSC traces from Bai3 knockout PCs (left: CTRL, right: +C1ql1). G Total CF-EPSC amplitude. The graph shows the sum of peak amplitudes of single CF-EPSCs or multiple CF-EPSCs. p = 0.8534, two-tailed Welch’s t-test; n = 31 cells from 3 mice (CTRL); n = 42 cells from 4 mice (+ C1ql1). H The percentage of the number of CFs innervating single PCs. p = 0.6159, Mann–Whitney U test; n = 38 cells from 3 mice (CTRL); n = 50 cells from 4 mice (+ C1ql1). I Average of the 10–90% rise time of CF-EPSCs. p = 0.0609, two-tailed Welch’s t-test; n = 33 responses from 3 mice (CTRL); n = 46 responses from 4 mice (+ C1ql1). Bars represent mean ± SEM. **p < 0.01; *p < 0.05; ns, not significantBack to article page