Fig. 5
Endogenous Bai3 is required for synapse formation of re-innervating CFs
A Experimental scheme. B Representative CF-EPSC traces recorded from PCs of the indicated genotypes. C Total CF-EPSC amplitude. The graph shows the sum of peak amplitudes of single CF-EPSCs or multiple CF-EPSCs. CTRL (n = 17 cells from 2 mice) vs. Bai3 knockout (KO), p = 1.272 × 10− 6 (n = 29 cells from 5 mice); vs. GluD2 KO, p = 0.8311 (n = 25 cells from 5 mice); vs. Bai3 KO::GluD2 KO, p = 1.336 × 10− 6 (n = 33 cells from 4 mice). One way ANOVA followed by Dunnett’s test. D The percentages of the number of CFs innervating single PCs. The number of EPSCs evoked by distinct CF activation thresholds (step numbers) is shown. CTRL (n = 29 cells from 2 mice) vs. Bai3 KO, p = 0.9859 (n = 49 cells from 5 mice); vs. GluD2 KO, p = 1.885 × 10− 5 (n = 46 cells from 5 mice); vs. Bai3 KO::GluD2 KO, p = 0.3750 (n = 61 cells from 4 mice). Kruskal-Wallis test followed by Steel test. Bars represent mean ± SEM. **p < 0.01; ns, not significant