Fig. 1

Joint mobilization reverses mechanical hypersensitivity induced by CCI. a Time course of the effect of a 9-min of acute ankle joint mobilization on mechanical hypersensitivity in neuropathic mice. Each point represents the mean of 5–7 animals, error bars denote S.E.M. Data are representative of 2 independent sets of experiments. Statistical analyses were performed by two-way ANOVA followed by a Tukey's test. Asterisks denote a significant difference of ***P < 0.001 when compared with the control group, and hashtags denote P < 0.001 for comparison with the sham-operated group. b Time-course of the effect of 9-min joint mobilization treatment in either Cav3.2 null mice or wild-type mice subjected to CCI. Each data point represents the mean of 5–6 mice and data are representative of 2 independent sets of experiments. This particular experiment was performed with the experimenter being blinded to genotype. Statistical analyses were performed by two-way ANOVA followed by a Tukey's test. Asterisks denote a significant difference of ***P < 0.001 when compared with the control group. c Time-course and (d) bar graph representing the effect of URB597 (10 mg/kg, i.p.) on nocifensive responses of neuropathic wild type and Cav3.2 null mice, expressed as area under the curve (A.U.C.). Each point or bar represents the mean of 5–6 mice, error bars are SEM. Data are representative of 2 independent sets of experiments. Statistical analyses were performed by two-way ANOVA followed by a Tukey's test. Symbols denote a significant difference of **P < 0.01, ***P < 0.001, or ****P < 0.0001 when compared with the control group