Fig. 3
From: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress
Evaluation of control and TDP-43 mutant-expressing neurons at approximately 60 days after seeding. A, D Three lines of unedited iPSCs and gene-edited iPSCs (WT and A382T lines) were differentiated into motor and sensory neurons. There were no differences in the morphology of motor neuron or sensory neuron bodies, axons, or dendrites among the three groups. TDP-43 was localized to the nucleus, and no aggregate formation was observed. B, E The efficiency of motor neuron differentiation was approximately 45%, as calculated by the ratio of ChAT-positive cells to cells with nuclear staining. The efficiency of sensory neuron differentiation was approximately 40%, as calculated by the ratio of Brn3a-positive cells to cells with nuclear staining. There were no significant differences in the percentage of cells positive for certain markers. C, F There were no significant differences in TDP-43 RNA levels in motor and sensory neurons among the three groups. Scale bars, 20 μm in A, D. B, C, E, F n = 3 in each of three groups. The data are presented as the mean ± SEM. n.s.: not significant. Statistical analyses were performed by one-way ANOVA