Fig. 5
From: Specific vulnerability of iPSC-derived motor neurons with TDP-43 gene mutation to oxidative stress
Evaluation of apoptosis induced by oxidative stress. A Conditions of hydrogen peroxide treatment and timing of CC3 expression evaluation. B, D Immunostaining of apoptotic neurons for the markers of apoptosis CC3. The SMI32/CC3-double positive cells in A382T iMN H2O2+ are indicated by arrowheads. C, E Quantitative analysis of the percentage of apoptotic neurons. The percentage of apoptotic motor neurons was higher in the A382T group than in the control group. There were no significant differences in the percentage of apoptotic sensory neurons. Scale bars, 20 μm in B and D. C, E n = 3 in each of the three groups. The data are presented as the mean ± SEM. n.s., not significant, *P < 0.05. Statistical analyses were performed by one-way ANOVA