Fig. 1

Contextual novelty-induced gene expression in the dorsal hippocampus in mice and rats. (A) Behavioral equipment for contextual novelty in mice. (B) Schematic overview of the mouse experimental design. Animals were divided into two groups in the critical session (Group CC, caged control; Group NOV, 5-min contextual novelty exploration; n = 5 in each group). Brain samples were collected 25 min after a 5-min novelty exploration, followed by laser microdissection of the hippocampal CA1 pyramidal cell layer and multiplexed gene expression analysis. (C) Behavioral equipment for contextual novelty in rats. (D) Schematic overview of rat experimental design. Animals were divided into three groups in the critical session (Group CC, caged control; Group NOV-Veh, 5-min contextual novelty exploration with Veh injection; Group NOV-SCH, 5-min novelty exploration with SCH 23390 injection; n = 10 in each group). Brain samples were collected 30 min after a 5-min novelty exploration, followed by dissection of the dorsal hippocampus and multiplexed gene expression analysis