Fig. 3
Rab11a-shRNA reverses CFA induced allodynia
A, Experimental procedure of Rab11a-shRNA application. B, immunofluorescent samples showing that Rab11a-shRNA reversed the increased number of Rab11a positive cells (C, n = 3 mice in each group, unpaired t-test, t = 3.558, P = 0.0236) and Fos positive cells (D, n = 3 mice in each group, unpaired t-test, t = 8.082, P = 0.0013) induced via CFA injection. E, Expression level of Rab11a in sham + scrambled-shRNA (sham + scramble), sham + Rab11a-shRNA (sham + shRNA), CFA + scramble, and CFA + shRNA groups. CFA injection increased Rab11a expression, which was significantly reversed by Rab11a-shRNA (n = 3 mice in each group, One-Way ANOVA followed by Tukey’s post-hoc test, F(3,8) = 14.27, P = 0.0014, **P < 0.01). F, PWMT of sham + scramble, CFA + scramble, sham + shRNA and CFA + shRNA groups. CFA injection reduced the PWMT, which was reversed by Rab11a-shRNA (n = 6 mice in each group, Two-Way ANOVA followed by Tukey’s post-hoc test, F(3,16) = 57.22, P < 0.0001). Sham + scramble vs. CFA + scramble, ###P < 0.001; sham + shRNA vs. CFA + shRNA, *P < 0.05, **P < 0.01. Bars equal to200 µm and 100 μm in B