Fig. 4
Rab11a-shRNA decreases the amplitude of NMDA receptor-mediated EPSC.
A, Representative trace of sEPSC in Sham + scrambled-shRNA (Sham + scramble, n = 13 cells of 3 mice), Sham + Rab11a-shRNA (Sham + shRNA, n = 13 cells of 3 mice), CFA + scrambled-shRNA (CFA + scramble, n = 12 cells of 3 mice), and CFA + Rab11a-shRNA (CFA + shRNA, n = 17 cells of 4 mice) groups. B, The frequency (One-Way ANOVA followed by Tukey’s post-hoc test, F(3,51) = 4.240, P = 0.0095, *P < 0.05) and amplitude (One-Way ANOVA followed by Tukey’s post-hoc test, F(3,55) = 5.411, P = 0.0025, **P < 0.01) of sEPSC. C, Representative traces of AMPA receptor mediated EPSC from Sham + scramble, Sham + shRNA, CFA + scramble, and CFA + shRNA groups. D, Averaged amplitude of AMPAR mediated EPSC from sham + scramble (n = 9 cells of 3 mice), sham + shRNA (n = 9 cells of 3 mice), CFA + scramble (n = 8 cells of 3 mice) and CFA + shRNA (n = 8 cells of 3 mice) groups (Two-Way ANOVA followed by Tukey’s post-hoc test, F(3,30) = 6.232, P = 0.0020). CFA increased the amplitude of NMDA receptor mediated EPSC when voltage of 5 V (P = 0.0294), 6 V (P = 0.0447), 7 V (P = 0.0494) and 8 V (P = 0.0143) were applied. However, the shRNA injection group did not produce a significantly lower amplitude compared with the CFA + scramble group. E, Representative traces of NMDA receptor mediated EPSC from Sham + scramble, Sham + shRNA, CFA + scramble, and CFA + shRNA groups. F, Averaged amplitude of NMDAR mediated EPSC from sham + scramble (n = 8 cells of 3 mice), sham + shRNA (n = 9 cells of 3 mice), CFA + scramble (n = 9 cells of 3 mice) and CFA + shRNA (n = 10 cells of 3 mice) groups (Two-Way ANOVA followed by Tukey’s post-hoc test, F(3,32) = 4.901, P = 0.0065, *P < 0.05). CFA increased the amplitude of NMDA receptor mediated EPSC when voltage of 7 V (P = 0.0409) and 8 V (P = 0.0284) were applied, and the shRNA injection group produced a significantly lower amplitude in response to voltage of 7 V (P = 0.0417) and 8 V (P < 0.0310) compared with the CFA + scramble group