Fig. 3

Optogenetic inhibition of the ACC during conditioning reduces catastrophizing behavior. A Behavioral assay of eNpHR and eYFP rats. Yellow light treatment was paired with the vF chamber during conditioning. B After yellow light treatment to the ACC during conditioning, eNpHR rats no longer showed an aversion to the vF chamber (p = 0.6251, paired t test; n = 6 animals) (Preconditioning NS: 298.0 ± 32.35, Preconditioning vF: 302.0 ± 32.35, Testing NS: 324.6 ± 70.36, Testing vF: 275.3 ± 70.36). C After yellow light treatment to the ACC during conditioning, eYFP rats continued to exhibit an aversion to the vF chamber (*p < 0.05, paired t test; n = 6 animals) (Preconditioning NS: 299.0 ± 26.53, Preconditioning vF: 301.0 ± 26.53, Testing NS: 496.5 ± 38.50, Testing vF: 103.5 ± 38.50). D Yellow light treatment during conditioning reduced eNpHR rats’ aversion to the vF stimulus, relative to no light treatment (*p < 0.05, paired t test; n = 6 animals) (No Light: 239.3 ± 17.89, Yellow Light: 26.58 ± 51.10). E Yellow light treatment during conditioning did not reduce eYFP rats’ aversion to the vF stimulus, compared to no light treatment (p = 0.4539, paired t test; n = 6 animals) (No Light: 146.6 ± 49.03, Yellow Light: 197.5 ± 52.27). Data are represented as mean ± SEM