Fig. 4
From: AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo
Increasing Ca2+ in cultured hippocampal neurons through activation of monSTIM1 variants. A Schematic illustrating a protocol for light illumination. B Fluorescence images of RGECO1 in neurons co-expressing each monSTIM1 variant, illuminated with blue light (top). Summary data showing relative changes in RGECO1 intensity over time upon repeated light illumination (bottom). C Summary data showing maximum fold changes (ΔF/F0) in RGECO1 intensity upon activation of monSTIM1 variants. EGFP-monSTIM1: n = 6; FLAG-monSTIM1: n = 5; EGFP-CRY2-STIM1(318–450): n = 7; EGFP-IRES2-CRY2-STIM1(238–448): n = 7; EGFP-STIM1 + vhhGFP-CRY2: n = 5; CIBN-STIM1 + CRY2: n = 6 cells. Data are presented as means ± SEM (one-way ANOVA followed by multiple comparison test); ns, not significant (p > 0.05)