Fig. 7
From: AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo
Application of monSTIM1 variants in astrocytes. A Representative images showing FLAG-monSTIM1 expressed under the control of a GfaABC1D promoter targeted to the hippocampal CA1 region. Green, FLAG; Red, c-Fos; Blue, DAPI. Scale bar, 100 μm. B Representative images showing cFos-positive cells expressing FLAG-monSTIM1, with or without non-invasive light delivery. Green, FLAG; Blue, DAPI; Magenta, GFAP; Red, c-Fos. Scale bar, 50 μm. C Quantification of cFos-positive cells expressing FLAG-monSTIM1 in CA1 astrocytes. FLAG-monSTIM1 (-Light): n = 5; FLAG-monSTIM1 (+ Light): n = 6 mice. Data are presented as means ± SEM (*p < 0.05, **p < 0.01; Unpaired student’s t test); ns, not significant (p > 0.05)