Table 1 Sizes of DNA coding sequences for mammalian expression vectors and DNA sequences between ITRs in AAV vectors
From: AAV-compatible optogenetic tools for activating endogenous calcium channels in vivo
Mammalian expression vectors | Size (bp) |
|---|---|
Single component | |
EGFP-CRY2-STIM1(238–685) | 3555 |
HA-CRY2-STIM1(238–685) | 2817 |
FLAG-CRY2-STIM1(238–685) | 2868 |
EGFP-CRY2-STIM1(318–450) | 2610 |
EGFP-IRES2-CRY2-STIM1(238-448) | 3432 |
Two components | |
vhhGFP-CRY2 | 1920 |
EGFP-CRY2(238–685) | 2061 |
CIBN-STIM1(238–685) | 1854 |
CRY2 | 1494 |
AAV expression vectors | Size (bp) spanning ITRs |
|---|---|
AAV-CaMKIIα-FLAG-STIM1(238–685) | 4147 |
AAV-CaMKIIα-EGFP-CRY2-STIM1(318–450) | 3864 |
AAV-GfaABC1D-FLAG-STIM1(238–685) | 4467 |