Fig. 1
From: Limitations of the human iPSC-derived neuron model for early-onset Alzheimer’s disease
A Non-demented controls (NDCs) and EOAD iPSCs were differentiated using dual SMAD inhibitors (first step) and basic-FGF withdrawal (second step). B Metadata for NDC and EOAD subjects used in this study. C Multi-dimensional scaling (MDS) analysis after batch correction by experimental condition, sex and sequencing batch of filtered normalized RNA-seq data. D RNA-seq volcano plot of differentially expressed genes (DEGs) for EOAD patients relative to all NDCs as determined by kimma with an FDR p-value < 0.05. E Quasi-proportional Venn diagram overlap of DEGs across the four EOAD patients relative to all NDC subject neurons. F MDS (left) and UMAP (right) analysis based on NDC and EOAD neurons generated in this study using filtered, normalized RNA-seq data. G RNA expression profile heatmap corresponding to all filtered genes. H MDS (left) and UMAP (right) analysis based on NDC and EOAD neurons generated in this study using filtered, normalized RNA-seq data for neuron lineage genes. I RNA expression profile heatmap corresponding to either neuron lineage genes (left) or post-mitotic genes (right) clustered by subject experimental condition (NDC or EOAD)