Fig. 1

Expression time, but not injected volume, greatly impacts light-evoked potentials in the thalamus-amygdala pathway. A Schematic representation of the experimental timeline; B Example of tdTomato fluorescence tag (for AAV expression localisation) and approximate area of infection, for one hemisphere; C Schematic representation of the recording electrode positioning (left) and population field evoked responses based on the dorso-ventral profile observed during recording (middle) with a representative histological image showing the electrode lesion trace (right); D representative light-evoked fEPSP for different stimulation intensities; (E) quantification of approximate infection area based on expression time (x-axis) and injected volume (y-axis), n = 18 (n = 6 per WPI); F data as in E reorganised by expression time to show multiple comparisons across time points; G quantification of the amplitude of the evoked responses based on expression time and injected volume in a subset of the animals in C, n = 14 (4 WPI, n = 4; 6 WPI, n = 6; 8 WPI, n = 4); H data as in G reorganised by expression time to show multiple comparisons across time points. Data are represented as means and 95% coverage confidence intervals. Thick coloured lines represent statistically significant differences at a 5% level of significance. Thin grey lines represent no statistical significance detected. P-values are reported in Additional file 1: Figure S2