Fig. 4

ERs remarkably decrease and positively correlate with hyperlipidemia and cognitive impairment in ovariectomized LDLR ^−/− mice. C57BL/6 and LDLR^−/− mice were fed a ND or HFD for 90 days. Bilateral ovariectomy was performed in LDLR^−/− mice fed a HFD to simulate a postmenopausal stage. A Serum of mice was obtained after 90 days of administration, and the serum estradiol levels were detected using an ELISA kit. B The expression of ERα, ERβ and GPER in the hippocampal tissues of mice was detected by immunohistochemistry staining after 90 days of administration. The magnification and scale bar are marked in the representative images. C Related to B, the expression levels of ERα, ERβ and GPER in the hippocampus were quantified by mean optical density. D Pearson was used to conduct correlation analysis on serum E2 levels, hippocampal ERα, ERβ and GPER expression, hippocampal TC and TG levels, and cognitive behavior test results. The red background indicates a positive correlation, and the blue background indicates a negative correlation. The number in the circle indicates the correlation coefficient, and the symbol * in the circle indicates the significance. N = 5. In the indicated comparison, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001