Fig. 2

Piezo1 expression profiling, cellular mechanotransduction, and biomechanical correlation analysis across brain regions of different donors. The relative abundance of (A) Piezo1, (B) YAP, (C) pYAP, and (D) β-catenin in the regions of WM (magenta), GW junction (purple), and pons (green) for individual donors (D1-4). (E, top left) Spearman correlation between Piezo1 relative fluorescence units (RFU) and stiffness; GW Junction (orange) ns, WM (blue) r = -0.5341, p < 0.05, pons (green) ns. (E, top right) Correlation between Piezo1 regional RFU and spring term (\(\alpha\)); GW junction r= 0.8791, and WM r = -0.5341, p < 0.05. (E, bottom left) Correlation between Piezo1 regional RFU and decay term (b); WM r = -0.6758, and pons r = 0.7692, p < 0.05. (E, bottom right) Correlation between Piezo1 regional RFU and equilibrium stress term (\({\sigma }_{e})\); WM r = -0.8571, pons r = 0.7198, p < 0.05. (F) Comparison of protein expressed based on brain region for all donors, *p < 0.05