Fig. 4

Three-dimensional expression profile of Piezo1, Piezo2, TMEM150C across brain regions (WM, GW Junction, Pons) from four donors. (A-D) The top three rows represent the expression profile of Piezo1; the middle three rows represent the expression profile of Piezo2; the bottom three rows represent the expression profile of TMEM150C. (A-D) Columns represent the spatial position of each field of view showing the top, middle, and bottom sections of tissue in each brain region. Depth measurements for each field of view are labeled in the top right corner of each image (A-D). (A) expression analysis of and mechanosensor Piezo1, Piezo2, TMEM150C across brain regions (WM, GW Junction, Pons), (B) Donor 2 IF images for each brain region and mechanosensor, (C) Donor 3 IF images for each brain region and mechanosensor, (D) IF images for each brain region and mechanosensor Donor 4. (E) Anatomical legend for each brain region showing a coronal cross section (top) with an example of the spatial arrangement of each tissue layer for a particular brain region; the sagittal section (bottom) shows the spatial arrangement of each brain region. Scale bar = 50\(\mu\)m